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Journal: Frontiers in Immunology
Article Title: Novel prognostic signature unveils PSEN1 contributes to depression-induced lung adenocarcinoma progression
doi: 10.3389/fimmu.2026.1681306
Figure Lengend Snippet: PSEN1 induced by depression promotes LUAD proliferation. (A) Process of the animal experiment; (B) immobility times in the forced swimming test (FST) were compared between Con and CR mice (n=4); (C) total locomotion and central zone activity in OFT were compared between Con and CR mice (n=4); (D) total duration and migration to light box in LDBT were compared between Con and CR mice (n=4); (E) the LLC tumor growth curves and the end-point tumor weights and sizes were represented (n=4); (F) the mRNA levels of 14 DRGs in tumor tissues were tested by qRT-PCR (n=3); (G) immunofluorescence detection of PSEN1 protein expression in tumor tissues; (H) Validation of the overexpression of PSEN1 in A549 and H1299 cells by western blot; (I) validation of the overexpression of PSEN1 in A549 and H1299 cells by qRT-PCR; (J–L) determine the effect of PSEN1 overexpression on the proliferation of A549 and H1299 cells through CCK-8 (J) , colony formation assay (K) , and EdU (L) ; (M) survival curve of LUAD patients with low or high expression of PSEN1; (N) protein levels of PSEN1 in normal tissue and LUAD in CPATC database; (O) representative images ofimmunohistochemistry of PSEN1 in normal tissue and LUAD in HPA database. Data are represented as the mean ± standard error of the mean (SEM). *P < 0.05, **P < 0.01, ***P < 0.001, and ****P < 0.0001 by an unpaired Student’s t-test.
Article Snippet: After standard procedures for deparaffinization, rehydration, and blocking, the sections were subjected to overnight incubation at 4 °C with
Techniques: Activity Assay, Migration, Quantitative RT-PCR, Immunofluorescence, Expressing, Biomarker Discovery, Over Expression, Western Blot, CCK-8 Assay, Colony Assay